Isolation and purification of lyngbya majuscula on nutrient-enriched agar plates abstract lyngbya majuscula samples were obtained from the phycology laboratory stock culture of. Isolation and purification of lyngbya majuscula on nutrient-enriched agar plates isolation and purification of lyngbya majuscula on nutrient-enriched agar plates a special problem abstract lyngbya majuscula samples were obtained from the phycology laboratory stock culture of the upv institute of aquaculture. Abstract this work presents the characterization of an uptake hydrogenase from a marine filamentous nonheterocystous cyanobacterium, lyngbya majuscula ccap 1446/4 the structural genes encoding the uptake hydrogenase (hupsl) were isolated and characterized, and regulatory sequences were identified upstream of hupsin silico analysis highlighted various sets of long. Overall, this study is the first to report on apoptotic property of cyanobacterial phycoerythrin from marine lyngbya sp a09dm against a549 human lung carcinoma cells and requires a detailed scrutiny to establish its anti-cancer potential. Tropical parasitic and infectious diseases, such as leishmaniasis, pose enormous global health threats, but are largely neglected in commercial drug discovery programs however, the panama.
An analogue of the potent microfilamentdisrupter lyngbyabellin a (1) has been isolated as a minor metabolite from the marine cyanobacterium lyngbya majuscula collected at apra harbor, guam. Sanae n moikeha and george w chu, dermatitis-producing alga lyngby a majuscula gomont in hawaii ii ii biological properties of the toxic factor1,2 , journal of phycology , 7 , 1 , (8) , (1971). Abstract: the lipid extract of a madagascan lyngbya majuscula has yielded malyngamides q and r, both amides of 7-methoxytetradec-4-enoic acid the isolation of these metabolites was accomplished using preparative liquid chromatography, with final purification.
An abstract of the thesis of lik tong tan for the degree of doctor of philosophy in pharmacy presented on april collection of the marine cyanobacterium lyngbya majuscula gomont made in papua iv2 isolation and purification of compounds151 to156 100 iv3 111 nmr spectrum of clairamide b(152). Isolation, purification, and structure elucidation of the antiproliferative compound coibamide a mcphail k, medina r, gerwick w, goerger d, capeon t antimalarial linear lipopeptides from a panamanian strain of the marine cyanobacterium lyngbya majuscula mcphail kl, correa j, linington rg, gonzalez j, ortega-barría e, capson tl, gerwick wh. Fractionation of the extract of the marine cyanobacterium lyngbya majuscula collected from panama led to the isolation of malyngolide dimer (1) the planar structure of 1 was determined using 1d and 2d nmr spectroscopy and hresi-tofms. Isolation and structural elucidation of the cryostatic linear and cyclic depsipeptide dolastatin 16, dolastatin 17, and dolastatin 18. Genome assembly and annotation l majuscula 3l sequence reads were obtained from two independent, nonaxenic cultures, using two different dna isolation procedures and two different sequencing technologies (sanger and 454 approaches) the reads from both the sanger and 454 libraries were pooled and treated as a single metagenomic dataset to identify core sequences of the lyngbya.
Lyngbya species, especially l majuscula, are prolific producers of secondary metabolites (jones et al, 2009) although comparatively few secondary metabolites have been characterized from l aestuarii , the isolation of an herbicidal polyketide natural product has been reported ( entzeroth et al , 1985 . Structure and biosynthesis of the jamaicamides, new mixed polyketide-peptide neurotoxins from the marine cyanobacterium lyngbya majuscula chem biol 2004 11 : 817-833 view in article. Lagunamides a (1) and b (2) are potent cytotoxic cyclic depsipeptides isolated from the filamentous marine cyanobacterium, lyngbya majuscula, from pulau hantu, singapore these compounds are structurally related to the aurilide-class of molecules, which have been reported to possess exquisite antiproliferative activities against cancer cells.
Although isolation of pure compounds from difficult matrices like organic matter is still challenging and we are far from isolation procedures in one step, the application of more selective methods from extraction to fractionation and purification will speed up the time from collection of biological material to final purified compound. Lyngbya majuscula produces a variety of secondary pounds over time in addition, we either fed fish only fish scarus schlegeli to repeated preference tests with isolation of secondary metabolites then periodically subjected to hplc purification [[email protected] silica 10 pm, 250 x 22 mm column, 65% ethyl acetate:hexanes (v:v), refractive. isolation and purification of lyngbya majuscula on nutrient-enriched agar plates a special problem isolation and purification of lyngbya majuscula on nutrient-enriched agar plates abstract lyngbya majuscula samples were obtained from the phycology laboratory stock culture of the upv institute of aquaculture. We have pursued activity-guided isolation from an extract of lyngbya majuscula that was found to be active against leishmaniasis a new modified linear peptide from the dragonamide series was isolated, dragonamide e (1), along with two known modified linear peptides, dragonamide a (2.
Lyngbya majuscula strain jhb was collected in hector's bay, jamaica both cultures were subsequently isolated to a monoclonal culture by using standard microbiological isolation techniques both cultures were subsequently isolated to a monoclonal culture by using standard microbiological isolation techniques. Lyngbya majuscula the first report of an oxygenated fatty acid metabolite from a cyanobacterium came from work with lyngbya majuscula in 1978 (cardellina et al, 1978. Toxic lyngbya majuscula in coastal waters may represent a rather specific hazard, as there are several anecdotal reports and outbreak-initiated investigations of non-bathers at beachside locations suffering respiratory and ocular symptoms that were temporally related to the presence of l majuscula blooms an outbreak of upper respiratory and.